光遗传技术具有独特的高时空分辨率和细胞类型特异性两大特点，克服了传统手段控制细胞或有机体活动的许多缺点，能对神经元进行非侵入式的精准定位刺激操作而彻底改变了神经科学领域的研究状况，为神经科学提供了革命性的研究手段。该技术的应用在这两年得到飞速的发展，应用研究领域涵盖多个经典实验动物种系（果蝇、线虫、小鼠、大鼠、绒猴以及食蟹猴等），并涉及神经科学研究的多个方面，包括神经环路基础研究、学习记忆研究、成瘾性研究、运动障碍、睡眠障碍、帕金森症模型、抑郁症和焦虑症动物模型等应用。
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Simultaneous and precise illumination and ablation |
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| MicroPoint提供了一个灵活和区域可选的光刺激工具。通过脉冲氮泵浦可调谐染料激光器，可进行细胞损伤、DNA损伤以及365nm至656nm波长范围内的漂白和解笼锁实验。利用染料激光器可产生20多种激发波长，能量可控，通过二向色滤光片组，能够同时进行成像和光刺激 |
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| 此系统可配置在Leica, Nikon, Olympus and Zeiss.等显微镜系统中 |
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| 光谱范围 | 365 nm - 656 nm |
| 光强调节范围 | 0.1%-100% |
| 光斑峰值功率 | 12 kW |
| 脉宽 | 3 - 5 nsec |
心血管药物研发 |
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| 利用MicroPoint 高能激光脉冲造成小鼠脑部血栓，观察血栓形成及用药后溶栓的过程 Representative composite photomicrographs of fluorescent images depicting human thrombusformation in homozygous mutant (upper panels) or WT (lower panels)mice (V, venule; A, arteriole). Rhodamine and BCECF are depicted in red and green, respectively, and merged is presented in yellow. Modifying murine von Willebrand factor A1 domain for in vivo assessment of humanplatelet therapies J.C. Chen et al. Nature Biotechnology |
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| 胚胎发育调控 | |
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| 2. Similar cells in CD41: eGFP embryos previously injected with caged rhodamine were targeted 40 hpf with MicroPoint 365 nm pulses for 10-15 seconds each. Ten cells were targeted in each embryo |  |
| 3. T heir fate was tracked and four days later they can be seen to invade the thymus and become rag2: eGFP thymocytes. (Bertrand J Y et al. Development 2008;135: 1853-1862) |
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Invivo DNA定点损伤 |
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应用示例：（Micropiont 365nm脉冲激光细胞DNA DSB损伤研究） DNA-PKCS accumulates at DSB sites induced by laser microirradiation and heavy charged particles. Coimmunostaining of microirradiated YFP-DNA-PKCS–expressing V3 cells with rH2AX antibody (blue) and phosphospecifi c antibodies to the Ser-2056 or Thr-2609 amino acid residues of DNA-PKCS (red). Bar, 10 um.Autophosphorylation of DNA-PKCS regulates its dynamics at DNA double-strand breaks Benjamin P.C.Chen,1 and David J. Chen1 |
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Copyright © 2008 ColdSpring Science Corporation. 冷泉科技有限公司 京ICP备05055950
光激活及光损伤系统
